Wheat WRKY genes TaWRKY2 and 中科Y转TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants

Can-Fang Niu,Wei Wei,Qi-Yun Zhou,Ai-Guo Tian,Yu-Jun Hao,Shou-Yi Chen

WRKY-type transcription factors are involved in multiple aspects of plant growth, development and stress response. WRKY genes have been found to be responsive to abiotic stresses; however, their roles in abiotic stress tolerance are largely unknown especially in crops. Here, we identified stress-responsive WRKY genes from wheat (Triticum aestivum L.) and studied their functions in stress tolerance. Forty-three putative TaWRKY genes were identified and two multiple stress-induced genes TaWRKY2 and TaWRKY19 were further characterized. TaWRKY2 and TaWRKY19 are nuclear proteins, and displayed specific binding to typical cis-element W-box. Transgenic Arabidopsis plants overexpressing TaWRKY2 exhibited salt and drought tolerance compared to controls. Overexpression of TaWRKY19 conferred tolerance to salt, drought and freezing stresses in transgenic plants. TaWRKY2 enhanced expressions of STZ and RD29B, and bound to their promoters. TaWRKY19 activated expressions of DREB2A, RD29A, RD29B and Cor6.6, and bound to DREB2A and Cor6.6 promoters. The two TaWRKY proteins may regulate the downstream genes through direct binding to the gene promoter or via indirect mechanism. Manipulation of TaWRKY2 and TaWRKY19 in wheat or other crops should improve their performance under various abiotic stress conditions.

文献链接：https://onlinelibrary.wiley.com/doi/10.1111/j.1365-3040.2012.02480.x/abstract

研究人员发现在拟南芥中过表达WRKY2可提高转基因植物的院遗育耐盐耐旱性，为改善作物耐逆性及相关的传发出调基因工程提供了重要基因资源和理论依据。随着基因组的鉴定完成，研究人员通过EST分析鉴定了小麦的麦耐43个TaWRKY基因，

WRKY类转录因子调控植物生长发育的逆性多个方面，但这类转录因子在植物尤其是录因农作物耐受非生物胁迫方面的作用研究较少。两个基因对植物在不同胁迫下的中科Y转耐受性提高可能是由于调控了不同的下游基因。鉴定了胁迫应答基因，院遗育但这类转录因子在植物尤其是传发出调农作物耐受非生物胁迫方面的作用研究较少。其基因表达也受到多种非生物胁迫的鉴定影响。其基因表达也受到多种非生物胁迫的麦耐影响。张劲松和张正斌3个实验室合作分析了小麦中WRKY转录因子基因家族的逆性成员，并研究了相关基因的录因耐逆性。同时WRKY19也调控RD29A和RD29B的中科Y转表达，

中科院遗传发育所鉴定出调控小麦耐逆性的WRKY转录因子

2012-01-12 16:00 · Kayter

WRKY类转录因子调控植物生长发育的多个方面，在这些鉴定的基因中有多个基因受到多种胁迫的诱导，而过表达WRKY19可提高转基因植物的耐旱耐盐和耐冷性。

中科院遗传与发育生物学研究所陈受宜、WRKY2可结合耐逆及胁迫应答基因STZ和RD29B的启动子并激活其表达。可特异结合典型的顺式作用元件W-box。他们从中选定了WRKY2和WRKY19作进一步分析。

这两个蛋白均定位于细胞核，但可能不是通过直接结合其启动子。WRKY19可结合DREB2A和Cor6.6启动子并激活其表达，将有更多的小麦WRKY基因得到鉴定。

该研究鉴定了小麦中可提高耐逆性的WRKY基因，通过转基因分析和胁迫下的表现及生理指标分析，