这些结果不仅仅揭示了水稻土中甲酸代谢微生物，稻田

Identification of Formate-Metabolizing Bacteria in Paddy Soil by DNA-Based Stable Isotope Probing

Youzhi Feng,土壤 Xiangui Lin, Zhongjun Jia and Jianguo Zhu

As one of the fermentation products of plant biomass, formate is an important organic acid in flooded rice (Oryza sativa L.) fields. Little is known, however, about the identity of formate-metabolizing bacteria in paddy soil. In this study, a paddy soil microcosm was incubated with 13C-labeled formate; bacterial and eukaryotic communities incorporating formate C were analyzed by DNA-stable isotope probing. Real-time quantitative polymerase chain reaction analysis revealed that 13C-formate-derived C was actively assimilated by a subset of bacteria. Molecular fingerprinting and sequencing analysis of 16S rRNA genes revealed that the active formate-metabolizing bacteria in the paddy soil were mainly the bacteria in the Clostridia class and purple nonsulfur bacteria (PNSB). The former might facilitate SO42− and Fe reduction using formate. Observed for the first time, PNSB are able to assimilate organic matter for growth under in situ conditions without light. These results extend our knowledge of not only the formate-assimilating bacteria but also the ecological significance of formate in paddy soil.

文献链接：https://www.soils.org/publications/sssaj/abstracts/76/1/121?access=0&view=pdf

全球稻田面积约1.5亿公顷，土壤甲酸是微生物研稻田有机质降解过程中重要中间产物之一，

SSSAJ：南京土壤所稻田土壤微生物研究获进展

全球稻田面积约1.5亿公顷，京土究获进展前者可利用甲酸为电子供体促进水稻土中硫酸盐和铁还原过程；紫色非硫细菌是稻田首次原位条件下被发现可在黑暗中有机异养生长。指纹图谱技术和系统发育分析，土壤厌氧环境使得水稻土含有丰富的小分子有机酸。微生物驱动着稻田土壤生物地球化学循环过程。但水稻土中甲酸代谢微生物及其生态功能却鲜有报道。获得甲酸代谢微生物13C-DNA；借助实时定量PCR技术、为全面理解微生物驱动下的土壤养分循环过程及其调控机制提供了重要科学依据。

中科院南京土壤研究所林先贵研究员团队通过稳定性同位素核酸探针技术，

该成果发表在Soil Science Society of America Journal上，微生物驱动着稻田土壤生物地球化学循环过程。让微生物“吃掉”甲酸的13C；再利用超高速密度梯度离心对土壤总DNA进行分层，通过代谢这些物质，利用13C标记的甲酸培育水稻土，清楚表明13C-甲酸被土壤梭菌和紫色非硫细菌利用。

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